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anti skp2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti skp2
    Anti Skp2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+skp2/Skp2+XP+Rabbit+mAb/10__1172_slash_jci195538-234-8-16
    Average 95 stars, based on 123 article reviews
    anti skp2 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    In Vivo:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Mutagenesis:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Transfection:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Quantitative RT-PCR:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Over Expression:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Knockdown:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    In Vitro:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Ubiquitin Proteomics:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Proliferation Assay:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Clonogenic Cell Survival Assay:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    shRNA:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Control:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Injection:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Expressing:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Staining:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Software:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Purification:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Binding Assay:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Selection:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Clone Assay:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Incubation:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Stable Transfection:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Immunohistochemical staining:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.

    Activation Assay:

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 C).. Database mining.Database mining.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 C); rabbit-b-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 C); mouse-b-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 C with antibody against FBXW2, b-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 C. The immunoprecipitates were washed with lysis buffer and assessed by IB.

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.Briefly, after deparaffinization, rehydration, antigen retrieval and blocking, the tissue slides were incubated overnight at 4 °C with indicated antibodies.. The following primary antibodies were used: rabbit-FBXW2 (ab83467, Abcam; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, Cellsignal; 1:500 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:1,000 overnight, 4 °C); mouse-ki-67 (#550609, BD Pharmingen; 1:1,000 overnight, 4 °C); rabbit-Cleaved Caspase-3 (Asp175) (#9661, Cellsignal; 1:500 overnight, 4 °C).

    Article Title: The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor
    Article Snippet: For direct IB analysis, cells were lysed in a Triton X-100 or RIPA buffer with phosphatase inhibitors.. The following primary antibodies were used: goat-FBXW2 (sc-160326, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (ab83467, Abcam; 1:1,000 overnight, 4 °C); rabbit-FBXW2 (#11499-1-AP, Proteintech; 1:500 overnight, 4 °C); rabbit-SKP2 (#2652, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-β-TrCP1 (D13F10) (#4394, cellsignal; 1:1,000 overnight, 4 °C); rat-HA (#11867423001, Roche Life Science; 1:2,000 overnight, 4 °C); mouse-FLAG (#F1804, Sigma; 1:2,000 overnight, 4 °C); mouse-P21 (#556430, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-P27 (#554069, BD Pharmingen; 1:2,000 overnight, 4 °C); mouse-β-Actin (sc-47778, SANTA CRUZ; 1:10,000 overnight, 4 °C); rabbit-Cleaved Notch1 (Val1744) (#4147, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-GRK2 (C-15) (sc-562, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-VRK2 (H-255)(sc-98733, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL11 (H-120)(sc-135126, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXL3 (GTX110755, GeneTex; 1:1,000 overnight, 4 °C); mouse-p130 (A-10) (sc-374521, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FBXW11 (#13149-1-AP, Proteintech; 1:1,000 overnight, 4 °C); rabbit-wee1 (#4936, Cellsignal; 1:1,000 overnight, 4 °C); rabbit-c-jun (sc-1694, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-cul-1(sc-11384, SANTA CRUZ; 1:1,000 overnight, 4 °C); rabbit-FOXO1 (#2880, Cellsignal; 1:1,000 overnight, 4 °C); mouse-cdh1 (#CC43, Calbiochem; 1:1,000 overnight, 4 °C); rabbit-rbx1 (made by our lab; 1:1,000 overnight, 4 °C).. To immunoprecipitate endogenous proteins, whole cell extracts were pre-cleared with normal IgG-AC (Santa Cruz) followed by overnight incubation at 4 °C with antibody against FBXW2, β-TrCP, FBXL3, FBXL11 or SKP2; to immunoprecipitate exogenously expressed FLAG-tagged or HA-tagged proteins, the pre-cleared cell lysates were incubated with bead-conjugated FLAG (Sigma) or with HA antibody (Roche) in a rotating incubator overnight at 4 °C.



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